FC 25X electrophoresis buffer.

FC 25X electrophoresis buffer is available in 0.5 and 1 liter packages. It has a higher separation efficiency for electrophoresis of DNA and RNA segments than conventional TAE and TBE buffers. The 1X FC electrophoresis buffer runs agarose and polyacrylamide gels 4 times faster than conventional TAE or TBE while the image of nucleic acid bands is significantly improved. FC is the preferred buffer for the analysis of PCR products and most laboratory needs. Its low conductivity allows its use with a higher voltage (200 V), which means higher separation speeds of DNA molecules. The FC solution is suitable for DNA separations from 20 to 3000 bases in standard agarose. Excellent resolution over 2000 bp is provided on 0.3-0.5% agarose gel. Use it in the same way as conventional electrophoresis solutions. Fluorescent dyes such as ethidium bromide can be applied during gel preparation while other dyes such as SYBRGreen are applied after the gel has been solidified.